Engineering of Helicobacter pylori L-asparaginase: characterization of two functionally distinct groups of mutants.
Bacterial L-asparaginases have been used as anti-cancer drugs for over 4 decades though presenting, along with their therapeutic efficacy, several side effects due to their bacterial origin and, seemingly, to their secondary glutaminase activity.Helicobacter pylori type II L-asparaginase possesses interesting features, among which a reduced catalyt